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流式细胞术(flow cytometry,FCM)是一种快速对单细胞分析的新技术。我们应用该技术对门诊可疑食管癌患者的拉网细胞进行了DNA 定量测定,分析其DNA 倍体水平,细胞周期各时相细胞分布的变化,同时与细胞学检测进行比较,现将结果报道如下。材料与方法拉网细胞悬液取自门诊39例可疑食管癌患者,每例拉网标本分别做成流式细胞检测悬液样品和病理学细胞涂片。
Flow cytometry (FCM) is a new technique for rapid single cell analysis. We used this technique to perform quantitative DNA assays on pull-net cells in patients with suspected esophageal cancer in outpatients, analyzed their DNA ploidy levels, and cell cycle changes in cell distribution at each phase, and compared them with cytological tests. The results are reported below. . Materials and Methods Cell network suspensions were obtained from 39 patients suspected of esophageal cancer in the outpatient department. Each sample was made into a flow cytometric suspension sample and a pathological cell smear.