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报道了草莓花药培养以花蕾大小为标志的采样方法,以及适宜的培养基和培养方法。诱导第一、二级花序上直径5毫米以内花蕾的花药在 MS+BA0.25毫克/升+2,4—D0.25毫克/升培养基上产生愈伤组织,在 MS+BA0.5毫克/升培养基中分化、增殖、生根。诱导5—7毫米花蕾的花药则需要更高(0.5毫克/升)的外源激素 BA。对草莓花药培养和匍匐茎芽培养两种不同方式的作用和效果进行了比较,认为花药培养对无病毒苗的获得和快速繁殖有更好的效果。
Reported the strawberry anther culture bud size-based sampling method, as well as the appropriate medium and culture methods. The anthers inducing flower buds within 5 mm in diameter on the first and second inflorescences produce callus on MS + BA0.25 mg / L + 2,4-D0.25 mg / L medium in MS + BA 0.5 mg / L medium differentiation, proliferation, rooting. Anthers inducing 5-7 mm buds require a higher (0.5 mg / L) exogenous hormone, BA. The effects and the effects of two different ways of strawberry anther culture and stoloniferous bud culturing were compared. Anther culture was considered to have a better effect on virus-free seedling acquisition and rapid propagation.