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玉米株高不仅是一个重要的农艺性状,而且是发育生物学研究中较理想的模式性状之一.以优良玉米杂交组合综3×87-1的266个F_(2:3)家系为材料,利用覆盖玉米10条染色体的分子标记连锁图,采用复合区间作图法并结合条件分析法,在不同发育时期,研究玉米株高QTL的动态变化.通过一年两点(武汉和襄樊)的随机区组的田间实验,考察了5个不同时期的株高. 根据不同时期的实际数据进行QTL分析,在5条染色体的不同区段定位了8个QTL(LOD≥2.5),但在检测的时期和效应值上有一定的差异,有3个QTL在5个时期都检测到,不同的测定时期,单个QTL所解释的表型变异为3.8％～17.1％之间,表明控制株高的QTL在不同时期的表达是不一样的.根据不同时期的净增长量数据,共检测到7个控制株高的条件QTL,几乎在各个时期都检测到条件QTL,其中Ph1-1,Ph1-2,Ph3,Ph5-2和Ph9在2个点均同时被检测到.单个条件QTL所解释的表型变异为3.8％～12.3％之间.这些结果表明,控制玉米株高的QTL以一定的时空方式表达,因此,在对株高等数量性状进行分子标记辅助选择时,应对不同发育时期的QTL进行选择才可能达到理想效果.
The plant height is not only an important agronomic trait, but also one of the desirable model traits in developmental biology.Furthermore, 266 F 2 (3) families with 3 × 87-1 maize hybrids were used as materials, The molecular marker linkage map of 10 chromosomes in maize was used to study the dynamic changes of high QTL in maize at different developmental stages by using composite interval mapping and conditional analysis.After two random points (Wuhan and Xiangfan) According to field data from different periods, 8 QTLs (LOD≥2.5) were mapped in different segments of 5 chromosomes. However, during the period of detection And the effect value. There were 3 QTLs detected in 5 periods, and the phenotypic variation explained by a single QTL was between 3.8% and 17.1% in different measurement periods, indicating that the QTL for controlling plant height According to the data of net growth in different periods, seven QTLs for plant height were detected, and conditional QTLs were detected in almost all periods, of which Ph1-1, Ph1-2, Ph3 , Ph5-2 and Ph9 were detected at the same time in both sites The explained phenotypic variation ranged from 3.8% to 12.3% .These results indicated that the QTL controlling the plant height of maize was expressed in a certain spatiotemporal fashion, therefore, in the case of molecular marker-assisted selection of the quantitative traits of plant height, It is possible to achieve the desired effect by selecting the QTL for the period.