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目的 :研究微血管密度 (MVD)在声门上型喉鳞状细胞癌 (SGLSCC)中的分布及对预后的影响。方法 :应用CD31两步系统免疫组织化学染色方法研究 5 9例手术治疗的原发SGLSCC标本 ,并以 19例声带息肉(VCP)标本做良性对照。结果 :SGLSCC的间质中可见大量微血管存在 ,而VCP上皮下结缔组织中微血管数明显减少。Kendall等级相关分析表明 ,在SGLSCC各临床病理参数中 ,MVD仅与淋巴结转移有中等程度的正相关 (P <0 .0 1)。N0 与N+ 、SGLSCC与VCP间的MVD值差异经独立样本均数 t检验显示均有统计学意义 (均P <0 .0 1)。Kaplan Meier生存分析显示 ,MVD与SGLSCC患者术后累积生存率有统计学意义 (P <0 .0 1)。结论 :血管生成在肿瘤形成、侵袭与转移中有重要作用 ;MVD对颈淋巴结转移有预示作用 ;MVD与SGLSCC患者的预后有关。
Objective: To study the distribution of microvessel density (MVD) in supraglottic laryngeal squamous cell carcinoma (SGLSCC) and its effect on prognosis. Methods: Fifty-nine primary surgical specimens of SGLSCC were studied by CD31 two-step immunohistochemical staining and 19 cases of vocal cord polyp (VCP) specimens were used as benign controls. Results: A large number of microvessels were found in the interstitium of SGLSCC while the number of microvessels in subcutaneous connective tissue of VCP was significantly decreased. Kendall rank correlation analysis showed that MVD was only moderately positive correlated with lymph node metastasis in each clinicopathological parameters of SGLSCC (P <0.01). The differences in MVD values between N0 and N +, SGLSCC and VCP were all statistically significant (all P <0.01) by independent sample mean t test. Kaplan Meier survival analysis showed that postoperative cumulative survival rate of MVD and SGLSCC patients was statistically significant (P <0.01). Conclusion: Angiogenesis plays an important role in tumor formation, invasion and metastasis. MVD plays an important role in cervical lymph node metastasis. MVD is associated with the prognosis of patients with SGLSCC.