论文部分内容阅读
目的探讨泰淋方对慢性逆行性肾盂肾炎(CPN)肾皮质瘢痕产生的干预作用及其机制。方法将雄性SD大鼠随机分为正常组、模型组、左旋氧氟沙星对照组、泰淋方治疗组。建立逆行性CPN大鼠模型,分别予相应的药物干预。动态观察肾小管功能、肾功能、24h尿蛋白定量,以及造模侧肾脏的皮质瘢痕、病理形态学变化,并应用RT-PCR检测肾组织转化生长因子-β1(TGF-β1)mRNA的表达。结果①第30、60天各组尿N-乙酰-β-D-氨基葡萄糖苷酶/尿肌酐(NAG/Cr)无明显差异,而第90天模型组尿NAG/Cr显著升高,与两药物干预组相比差异有统计学意义(P<0.01);实验期间各组肾功能、24h尿蛋白无明显差异。②模型组肾皮质二级及以上瘢痕明显,大部分造模侧肾脏发生明显固缩、对侧代偿性增大,镜下见明显肾间质纤维化和小球周围纤维化;药物干预组瘢痕发生率显著减少(P(0.05),总体病变减轻。③模型组造模侧肾组织TGF-β1mRNA表达明显增加,而药物干预组明显回落。结论泰淋方能部分抑制细菌逆行感染导致的肾皮质瘢痕发生,并有效保护肾小管功能。
Objective To investigate the effect and mechanism of Tailing decoction on renal cortical scar formation in patients with chronic retrogressive pyelonephritis (CPN). Methods Male Sprague-Dawley rats were randomly divided into normal group, model group, levofloxacin control group and Tai Lin Fang treatment group. The establishment of retrograde CPN rat model, respectively, to the appropriate drug intervention. Renal tubular function, renal function, urinary protein excretion in 24h, cortical scar and pathomorphology were observed dynamically. The expression of transforming growth factor-β1 (TGF-β1) mRNA in renal tissue was detected by RT-PCR. Results ① There was no significant difference in urine NAG / Cr between the groups on the 30th and 60th days, while the urine NAG / Cr on the 90th day was significantly higher than that of the two groups Compared with the drug intervention group, the difference was statistically significant (P <0.01). During the experiment, the renal function and 24h urinary protein had no significant difference. ② In the model group, the scars on the secondary and above renal cortex were obvious. Most of the model kidneys were significantly compressed and contracted, with obvious renal interstitial fibrosis and peritubular fibrosis. The drug intervention group The incidence of scar reduced significantly (P (0.05), the overall lesion was alleviated.③The TGF-β1 mRNA expression in the model group was significantly increased, while the drug intervention group was significantly decreased.ConclusionTailing can partially inhibit the kidney caused by bacterial retrograde infection Cortical scarring occurs, and renal tubular function is effectively protected.