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硒为人体必需微量元素之一。生物样品中硒的测定有2,3-二氨基萘荧光光度法,阳极溶出伏安法,原子吸收吸光光度法,氢化物原子荧光法等。 SeSO_3~(-2)KIO_3体系催化极谱法测定微量硒有灵敏度高,选择性好,简便的优点,但有机物吸附于汞滴表面,改变汞滴表面性质,使催化电流受到影响,故此法成功运用于生物样品所见不多。何成杰等用此体系测定面粉和大米粉中的硒,但标准加入法不适宜大批生物样品的测试。我们采用钼盐作催化剂,催化消化全血样品,可明显加速高氯酸对有机物破坏,使有机物得以彻底破坏。试验取得了消解生物样品的最佳条件,同时进行了底液组份选择,使适合有
Selenium is one of the essential trace elements for the human body. Determination of biological samples of selenium with 2,3 - diaminonaphthalene fluorophotometry, anodic stripping voltammetry, atomic absorption spectrophotometry, hydride atomic fluorescence spectrometry. SeSO_3 ~ (-2) KIO_3 system has the advantages of high sensitivity, good selectivity and simpleness for the determination of trace selenium by catalytic polarography. However, the adsorption of organics on the surface of mercury drop and the change of mercury drop surface property affect the catalytic current, Used in biological samples see much. He Chengjie such as the determination of flour and rice flour in this system using selenium, but the standard method of adding a large number of biological samples is not suitable for testing. We use molybdenum salt as a catalyst, catalytic digestion of whole blood samples, can significantly accelerate the perchloric acid destruction of organic matter, so that organic matter can be completely destroyed. Experiments have been made to digest the best biological samples, while the bottom liquid component selection, so that suitable