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利用生物素（ｂｉｏｔｉｎ－１６－ｄＵＴＰ）标记的中间偃麦草（Ｔｈｉｎｏｐｙｒｕｍｉｎｔｅｒｍｅｄｉｕｍ）基因组ＤＮＡ作探针，以未标记的普通小麦中国春基因组ＤＮＡ作封阻ＤＮＡ（ｂｌｏｃｋｉｎｇＤＮＡ），对３个抗黄矮病小麦新种质的体细胞染色体进行分子原位杂交。结果表明：抗性源于Ｌ１的抗黄矮病小麦新种质Ｙｗ６４２为小片段易位系，含４０条小麦染色体和２条小麦－中间偃麦草易位染色体，易位的中间偃麦草染色体片段位于小麦染色体的端部；染色体配对和抗性分析表明该种质为纯合易位系。抗性源于无芒中４的小麦新种质Ｈｗ２４０和Ｙｗ０６０遗传构成不同：Ｙｗ０６０为易位系，含４０条小麦染色体和２条小麦－中间偃麦草易位染色体；Ｈｗ２４０为代换易位系，含３８条小麦染色体、２条中间偃麦草染色体和２条小麦－中间偃麦草易位染色体。在这２个种质中，易位的中间偃麦草染色体片段均位于小麦染色体端部。
Thinopyrum intermedium genomic DNA labeled with biotin-16-dUTP was used as a probe, and unlabeled wheat genomic DNA was used as blocking DNA. New germplasm somatic chromosomes for in situ hybridization. The results showed that the resistance to yellow dwarf resistant wheat Yw642 was a small fragment translocation line, which contained 40 wheat chromosomes and 2 wheat-Thinopyrum intermedium translocation chromosomes, Located at the end of wheat chromosome; chromosome pairing and resistance analysis showed that the germplasm is homozygous translocation line. The genetic constitution of Hw240 and Yw060, a new wheat germplasm derived from Wumangzhong 4, was different: Yw060 was a translocation line containing 40 wheat chromosomes and 2 wheat-Thinopyrum intermedium translocation chromosomes; Hw240 was a putative translocation line , Contains 38 wheat chromosomes, 2 Thinopyrum intermedium chromosomes and 2 wheat - Thinopyrum intermedium translocation chromosomes. In these two germplasms, the translocated Th. Intermedium chromosome fragments are located at the end of the wheat chromosome.