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目的:观察长期中等强度运动后大鼠心肌金属硫蛋白(MT)含量的变化,探讨金属硫蛋白对心肌的保护作用。方法:选用健康雄性SD大鼠40只,体重220～250g,随机分为安静对照组(A组);运动训练安静组(B组);中等强度运动训练(90分钟)+力竭组(C组);一次性力竭运动组(D组),每组10只。B、C组大鼠进行为期8周的中等强度运动。实验8周后,力竭组大鼠进行一次力竭运动。测定大鼠心肌组织中的MT含量、MDA含量和-SH含量。结果:(1)运动训练安静组和中等强度运动训练+力竭组大鼠心肌组织中MDA含量显著低于安静对照组(P<0.05),一次力竭运动组大鼠MDA显著高于安静对照组及运动训练安静组(P<0.05);(2)中等强度运动训练+力竭组大鼠-SH含量显著高于安静对照组和运动训练安静组(P<0.05),一次力竭运动组大鼠-SH含量显著低于安静对照组和运动训练安静组(P<0.05);(3)运动训练安静组和中等强度运动训练+力竭组大鼠MT含量显著高于安静对照组(P<0.05),且中等强度运动训练+力竭组显著高于运动训练安静组(P<0.05),一次力竭运动组大鼠MT含量显著低于安静对照组和运动训练安静组(P<0.05)。结论:运动可诱导MT合成。中等强度运动可促进心肌中MT合成适应性增加,提高心肌的抗氧化能力。
OBJECTIVE: To observe the change of myocardial MT content in long-term moderate-intensity exercise and to explore the protective effect of metallothionein on myocardium. Methods: Forty healthy male Sprague Dawley rats weighing 220-250 g were randomly divided into 4 groups: control group (group A), exercise group (group B), middle-intensity exercise group (90 minutes) Group); one-time exhaustive exercise group (group D), 10 rats in each group. B, C group of rats for 8 weeks of moderate-intensity exercise. Eight weeks after the experiment, exhaustive rats were subjected to a exhaustive exercise. The contents of MT, MDA and -SH in myocardium of rats were measured. Results: (1) The content of MDA in myocardial tissue of exercise-training quiet group and moderate-intensity exercise training + exhaustion group was significantly lower than that of quiet control group (P <0.05), the MDA content of exhaustive exercise group was significantly higher than that of quiet control group (P <0.05); (2) The content of -SH in moderate-intensity exercise training group and exhaustive exercise group was significantly higher than that in quiet control group and exercise-training quiet group (P <0.05) (P <0.05); (3) MT content of rats in exercise-training quiet group and moderate-intensity exercise training + exhaustion group was significantly higher than that in quiet control group (P < (P <0.05). The MT level of rats in exhaustive exercise group was significantly lower than that in quiet control group and exercise training group (P <0.05) ). Conclusion: Exercise can induce MT synthesis. Moderate-intensity exercise can promote the adaptation of myocardial MT synthesis increased, improve myocardial antioxidant capacity.