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目的前脑缺血再灌注可引起大鼠海马迟发性神经元死亡。但若在致死性缺血前，预先给于短暂的非致死性缺血刺激，即缺血坝处理（ｉｓｃｈｅｍｉｃ ｐｒｅｃｏｎｄｉｔｉｏｎｉｎｇ，ＩＰＣ），则可对其后的长时间致死性缺血产生耐受，此现象称为缺血耐受。ＨＳＰ_７０基因的转录被认为是细胞应激反应的基因标志。其基因产物ＨＳＰ_７０。具有细胞保护作用。本研究旨在探讨缺血预处理引起的缺血耐受中 ＨＳＰ_７０表达的变化。方法（１）建立动物模型：选用健康成年雄性Ｗｉｓｔａｒ大鼠，参照Ｐｕｌｓｉｎｅｌｌｉ的四血管阻断法建立前脑缺血模型。动物分三组：单纯缺血再灌注组（ＩＲ组），给予假手术３ｄ后行８ｍｉｎ致死性缺血；缺血预处理组（ＩＰＣ组），先给予３ｍｉｎ非致死性缺血．３ｄ后再给予８ｍｉｎ缺血：对照组，只给于假手术，不给予缺血刺激，（２）免疫投化检测；十缺血再灌注２ｈ，取脑制备冰冻切片，用ＳＡＢＣ 法检测各组海马ＣＡ_１区神经元ＨＳＰ_７０的表达。（３）流氏细胞术检测：于缺血再灌庄２４ｈ，迅速取而背侧新鲜海马组织，用流氏细胞仪检测海马ＣＡ_１区神经元ＨＳＰ_７０的阳性表达率。（４）病理学检查：再灌注７ｄ时取脑制备石蜡切片，行尼氏染色，光镜下观察海马ＣＡ
Objective Forebrain ischemia-reperfusion can cause delayed neuronal death in the hippocampus of rats. However, given a brief, non-fatal ischemic preconditioning (IPC) of ischemic preconditioning prior to lethal ischemia, this can be tolerated by subsequent long-term lethal ischemia, Phenomenon known as ischemic tolerance. The transcription of HSP70 gene is considered as a genetic marker of cellular stress response. Its gene product HSP_70. With cytoprotective effect. This study was designed to investigate the changes of HSP70 expression in ischemic tolerance caused by ischemic preconditioning. Methods (1) Establishment of animal model: Healthy adult male Wistar rats were selected, and the forebrain ischemia model was established according to the four-vessel occlusion method of Pulsinelli. The animals were divided into three groups: ischemia-reperfusion group (IR group), which were given 8-day lethal ischemia after 3-day sham operation; and ischemic preconditioning group (IPC group). 3min and then given 8min ischemia: control group, only given sham surgery, not given ischemia stimulation, (2) immunization detection; ten ischemia reperfusion 2h, take the brain to prepare frozen sections, SABC test groups The expression of HSP70 in neurons of hippocampal CA1 region. (3) Flow cytometry: Fresh hippocampus was rapidly removed from the dorsal side of ischemia-reperfusion rats 24h and the hippocampal CA1 neurons HSP70 positive rate was detected by flow cytometry. (4) pathological examination: 7d reperfusion brain preparation of paraffin sections, line Nissl staining, light microscopy, hippocampal CA