将非放射性标记物地高辛甙元用随机引物法由ｐＢＲ３２２－２ＨＢＶ重组质粒／ＥｃｏＲⅠ酶切片段（３．２ｋｂ）制备了乙型肝炎病毒ＤＮＡ探针。用斑点杂交、Ｓｏｕｔｈｅｒｎ杂交、组织原位杂交技术，结合乙肝血清学指标、ｓＩＬ－２Ｒ测定等综合分析了慢性乙型肝炎１７８例及肝细胞肝癌５４例的肝细胞、血清、淋巴细胞、唾液内ＨＢＶＤＮＡ的存在类型、复制表达状态、分布定位特点与病变发展趋势的相关性，探讨了肝癌高危人群肝组织可能发生恶性转化的预测信号及发病机理。 Hepatitis B virus DNA probe was prepared from non-radioactive marker digoxigenin by random primer method from pBR322-2HBV recombinant plasmid/EcoRI fragment (3.2kb). A total of 178 patients with chronic hepatitis B and 54 patients with hepatocellular carcinoma, including hepatocytes, serum, lymphocytes, and saliva, were analyzed using dot blot, Southern blot, and tissue in situ hybridization techniques combined with hepatitis B serological markers and sIL-2R assays. The correlation of the existence type, replication and expression status, distribution and location characteristics of HBVDNA with the development trend of the disease, and explored the predictive signal and pathogenesis of malignant transformation of hepatocellular carcinoma in high-risk populations.