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目的：探讨Ｐ物质（ＳＰ），神经肽Ｙ（ＮＰＹ）在分泌性中耳炎发病过程中的作用．方法：阻塞咽鼓管建立分泌性中耳炎模型．用免疫组化ＡＢＣ－ＧＤＮ及图像分析技术研究中耳粘膜ＳＰ，ＮＰＹ的分布及含量的变化．结果：中耳粘膜ＳＰ免疫反应（ＳＰ－ＩＲ）阳性纤维定位分布多与血管无关，ＮＰＹ免疫反应（ＮＰＹ－ＩＲ）阳性纤维多伴中小动脉走行．咽鼓管阻塞８ｈ后，中耳即有明确渗出，中耳粘膜ＳＰ－ＩＲ阳性纤维２４ｈ开始增粗、数量增多，至７２ｈ增多更明显，染色强度加深．７ｄ组中耳粘膜ＳＰ阳性纤维仍明显增粗，数量与正常无显著差别．中耳粘膜ＳＰ－ＩＲ阳性产物吸光度变化与渗液量呈正相关（ｒ＝０．９７５４，Ｐ＜０．０５），分泌性中耳炎对中耳粘膜ＮＰＹ－ＩＲ似无影响．结论：ＳＰ可能是分泌性中耳炎的重要炎性介质，中耳积液可能与ＮＰＹ无关．
Objective: To investigate the role of substance P (SP) and neuropeptide Y (NPY) in the pathogenesis of secretory otitis media. Methods: Eustachian tube was obstructed to establish the secretory otitis media model. The immunohistochemical ABC-GDN and image analysis techniques were used to study the distribution and content of SP and NPY in the middle ear mucosa. Results: The localization of SP-IR positive fibers in the middle ear mucosa was mostly unrelated to blood vessels. NPY-IR positive fibers were mostly accompanied by the arterioles. Eustachian tube obstruction 8h, the middle ear that there is a clear exudation, middle ear mucosa SP-IR positive fibers began to thicker 24h, an increase of more to 72h, staining intensity deepened. 7d middle ear mucosa SP positive fibers were still significantly thickening, the number was no significant difference with the normal. The change of absorbance of SP-IR positive product in middle ear mucosa was positively correlated with exudate (r = 0.9754, P <0.05), and secretory otitis media had no effect on NPY-IR of middle ear mucosa. Conclusion: SP may be an important inflammatory mediator of secretory otitis media, and middle ear effusion may not be related to NPY.