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目的:探讨益气补肾方法对于乳腺癌骨转移的抑制作用及其作用的机制。方法:将雌性Balb/c裸鼠随机分为治疗组和对照组,采用人乳腺癌细胞株MDA-MB-231注射裸鼠左心室,制作乳腺癌动物模型,分别予以益气补肾中药及生理盐水口服,通过骨ECT检查及病理检查明确其骨转移情况,然后通过免疫组化法,分别测定每组裸鼠血清中BSP、OPN,MMP-1的表达情况。结果:对照组的裸鼠骨转移发生率为80%,而治疗组的发生率为46.67%,与对照组比较,治疗组中裸鼠骨转移的发生率要明显降低;通过免疫组化检测,治疗组中的MMP-1和OPN的表达要明显低于对照组(P<0.05),治疗组中BSP的表达也要明显低于对照组(P<0.01)。结论:益气补肾中药能有效减少乳腺癌骨转移裸鼠模型的骨转移发生率,其作用机制可能为:通过对MMP-1,BSP,OPN的表达抑制,减少了乳腺癌细胞的脱落、迁移,并最终通过OPG-RANKL-RANK系统,抑制了破骨细胞的分化和骨的重吸收,减少了乳腺癌的转移和骨组织的破坏。
OBJECTIVE: To investigate the inhibitory effect and its mechanism of supplementing qi and tonifying kidney on bone metastasis of breast cancer. Methods: Female Balb / c nude mice were randomly divided into treatment group and control group. The human breast cancer cell line MDA-MB-231 was injected into the left ventricle of nude mice to make animal model of breast cancer. Oral, through the bone ECT examination and pathological examination to determine the bone metastasis, and then by immunohistochemistry, respectively, each group of nude mice serum BSP, OPN, MMP-1 expression. Results: The incidence of bone metastasis in control group was 80%, while the incidence of treatment group was 46.67%. Compared with the control group, the incidence of bone metastasis in nude mice in the treatment group was significantly reduced. By immunohistochemistry, The expression of MMP-1 and OPN in the treatment group was significantly lower than that in the control group (P <0.05). The expression of BSP in the treatment group was also significantly lower than that in the control group (P <0.01). Conclusion: Yiqi Bushen Chinese herbs can effectively reduce the incidence of bone metastasis in the nude mice model of bone metastasis of breast cancer. The possible mechanism is that the expression of MMP-1, BSP and OPN can reduce the shedding and migration of breast cancer cells , And finally through the OPG-RANKL-RANK system, inhibited osteoclast differentiation and bone resorption, reducing breast cancer metastasis and bone tissue destruction.