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目的:探讨霉酚酸酯(mycophenolate mofetil,MMF)对非肥胖性糖尿病小鼠(non-obese diabetic mice,NOD)胰岛炎和胰岛β细胞凋亡的保护作用。方法:8周龄NOD雌性小鼠22只分为两组,每组11只,干预组给予霉酚酸酯30mg/kg灌胃,对照组给予等量的生理盐水。两组小鼠饲养期间定期监测其血糖水平。30天实验结束时,每组取5只小鼠处死,行HE染色观察胰岛炎程度,TUNEL法检测胰岛β细胞凋亡率。余下小鼠观察到发生糖尿病或20周处死。结果:干预前两组小鼠血糖均在正常水平。干预期间和干预期满后,MMF干预组小鼠血糖与生理盐水对照组之间无差异(P>0.05);MMF组胰岛炎严重程度及胰岛β细胞凋亡率较生理盐水对照组明显减轻(P<0.01)。结论:MMF可以在早期抑制NOD小鼠的胰岛炎和胰岛β细胞凋亡,从而减轻胰腺损伤。如果在糖尿病发病前给予MMF可能延缓或防止糖尿病的发生。
Objective: To investigate the protective effect of mycophenolate mofetil (MMF) on the insulitis and β-cell apoptosis in non-obese diabetic mice (NOD). Methods: Twenty-two NOD female mice aged 8 weeks were divided into two groups with 11 rats in each group. The intervention group was given mycophenolate mofetil 30mg / kg, while the control group was given the same amount of saline. The two groups of mice were monitored regularly during their blood glucose levels. At the end of the 30-day experiment, 5 mice in each group were sacrificed and the degree of insulitis was observed by HE staining. The apoptosis rate of pancreatic β-cell was detected by TUNEL method. The remaining mice were observed with diabetes or 20 weeks of sacrifice. Results: Before the intervention, the blood glucose of the two groups of mice were normal. There was no significant difference between the MMF intervention group and the control group (P> 0.05). The severity of insulitis and the rate of pancreatic β-cell apoptosis in the MMF group were significantly reduced compared with the saline control group P <0.01). CONCLUSION: MMF can inhibit the insulitis and the islet β-cell apoptosis of NOD mice in early stage and relieve the pancreatic injury. If given before the onset of diabetes MMF may delay or prevent the occurrence of diabetes.