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肌动蛋白解聚因子(ADF)具有调节肌动蛋白解聚,促进丝极迅速转换的功能,在不同物种间存有同源性。但球虫目寄生虫类的研究未见报道。以刚地弓形虫RH株为实验对象,所有DNA操作流程均按Sambrook介绍法;双脱氧链终止法检测双链DNA序列。ADF质粒的构建:寡核苷酸5′-
Actin depolymerization factor (ADF) has the function of regulating actin depolymerization and promoting the rapid switch of filament, and has the homology among different species. However, there is no report on the species of coccidian parasites. Toxoplasma gondii RH strain was used as the experimental object. All DNA procedures were based on the Sambrook method. Double-stranded DNA was detected by dideoxy chain termination method. Construction of ADF plasmids: Oligonucleotides 5’-