论文部分内容阅读
为探讨锆对小鼠肝脏功能和病理组织学的影响,将50只健康6~8周龄清洁级雄性昆明小鼠按体重随机分成5组,分别为对照(生理盐水)组和0.855 mg/kg(1/200 LD50)、1.71 mg/kg(1/100 LD50)、3.42 mg/kg(1/50 LD50)、17.1 mg/kg(1/10LD50)氧氯化锆染毒组,每组10只。采用腹腔注射方式进行染毒,每日1次,连续30 d。测定血清谷氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)和乳酸脱氢酶(LDH)的含量及肝脏匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)的活力和丙二醛(MDA)的含量。结果显示,与对照组比较,仅17.1 mg/kg氧氯化锆染毒组小鼠血清中ALT含量升高(P<0.05);各剂量氧氯化锆染毒组小鼠血清中AST、LDH含量无明显变化;各剂量氧氯化锆染毒小鼠肝脏匀浆中GSH-Px活力及17.1 mg/kg氧氯化锆染毒组小鼠肝脏匀浆中SOD活力均下降,1.71、3.42、17.1 mg/kg剂量组小鼠肝脏匀浆中MDA含量均升高,差异有统计学意义(P<0.05)。表明在本实验范围内,仅17.1 mg/kg剂量组氧氯化锆对小鼠肝脏有轻微损伤。
To investigate the effect of zirconium on liver function and histopathology in mice, 50 healthy male Kunming mice, aged 6-8 weeks, were randomly divided into five groups according to body weight: control group (saline) and 0.855 mg / kg (1/200 LD50), 1.71 mg / kg (1/100 LD50), 3.42 mg / kg (1/50 LD50) and 17.1 mg / kg (1/10 LD50) . The rats were injected intraperitoneally once daily for 30 days. The levels of serum glutamate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) and the content of superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) activity and malondialdehyde (MDA) content. The results showed that compared with the control group, the serum ALT level increased only in 17.1 mg / kg oxychloride-treated mice (P <0.05). The levels of AST, LDH The activity of GSH-Px in liver homogenates and the activity of SOD in hepatic homogenates of mice treated with 17.1 mg / kg oxychloride zirconium chloride all decreased, while the activity of GSH-Px in liver homogenates decreased by 1.71,3.42, The content of MDA in hepatic homogenate of 17.1 mg / kg dose group was increased, the difference was statistically significant (P <0.05). In the experimental range, only zirconium oxychloride at a dose of 17.1 mg / kg showed slight damage to the liver of mice.