本文首先建立了血小板活化因子（ＰＡＦ）的生物活性检测法，并通过体外细胞培养技术，测定了肾小球系膜细胞在经ＬＰＳ、ＩＬ-１β、ＩＬ-６和ＴＮＦα的短暂刺激后，其培养上清中ＰＡＦ的活性。结果发现，它们均能诱导系膜细胞合成和分泌ＰＡＦ，ＩＬ-１β、ＩＬ-６和ＴＮＦα的诱生作用并呈一定的剂量依赖关系。实验提示上述因子在肾小球免疫病理损伤中，除直接作用外，也可通过诱生ＰＡＦ而间接发挥作用。应用ＰＡＦ拮抗剂来阻断这一途径，可能具有一定的临床应用价值。 This article first established a bioactive assay of platelet-activating factor (PAF), and measured in vitro cell culture techniques, mesangial cells were transiently stimulated by LPS, IL-1β, IL-6 and TNFα The culture supernatant PAF activity. The results showed that both of them can induce mesangial cells to secrete and secrete PAF, IL-1β, IL-6 and TNFα induced by a certain dose-dependent manner. Experimental results suggest that the above factors in the glomerular immune pathological damage, in addition to a direct role, but also indirectly induced by induced PAF. The use of PAF antagonists to block this pathway may have some clinical value.