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目的了解维吾尔族人群强直性脊柱炎(AS)患者人类白细胞抗原B27(HLA-B27)的表达状况。方法选择2010年1月-2014年12月新疆维吾尔自治区人民医院确诊的维吾尔族AS患者147例、疑似AS患者224例、正常人群100例作为研究对象。分别采集肘静脉血2 ml,遵照说明书进行处理后,经BD FacscantoⅡ流式细胞仪检测HLAB27抗原表达,并进行相应的统计分析。结果 471例研究对象中,共检出HLA-B27阳性244例,其中确诊AS组、疑似AS组和健康对照组HLA-B27检测阳性率分别为91.84%、46.88%和4.00%,且3组之间阳性率差异有统计学意义(P<0.01);确诊AS组和疑似AS组中男性HLA-B27阳性率均高于女性,且差异有统计学意义(P<0.05)。结论维吾尔族人群HLA-B27检测阳性率较高,临床可采用流式细胞术对维吾尔族人群开展常规HLA-B27检测,以实现AS早期发现,早期治疗。
Objective To investigate the expression of human leukocyte antigen B27 (HLA-B27) in Uygur ankylosing spondylitis (AS). Methods From January 2010 to December 2014, 147 cases of Uygur AS patients diagnosed in People’s Hospital of Xinjiang Uygur Autonomous Region, 224 cases of suspected AS patients and 100 cases of normal subjects were selected as the study subjects. The elbow venous blood were collected 2 ml, follow the instructions for treatment, the BD Facscanto Ⅱ flow cytometry HLAB27 antigen expression, and the corresponding statistical analysis. Results A total of 244 HLA-B27 positive cases were detected in 471 subjects. The positive rates of HLA-B27 in diagnosed AS group, suspected AS group and healthy control group were 91.84%, 46.88% and 4.00%, respectively. (P <0.01). The positive rate of HLA-B27 in diagnosed AS group and suspected AS group was higher than that in female group (P <0.05). Conclusions The positive rate of HLA-B27 in Uighur population is high. The routine HLA-B27 detection can be performed on Uighur population by flow cytometry in order to realize the early detection and early treatment of AS.