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Hjelm 等(1972)曾注意到与葡聚糖共价结合的蛋白 A 在层析纯化大多数哺乳类动物IgG 上的潜在用途。人 IgG_3不与蛋白 A 结合,将业经 DEAE 纯化的 IgG 加入蛋白 A-葡聚糖柱上,IgG_3单独出现在直接流出的组份中
Hjelm et al. (1972) have noted the potential use of Protein A covalently bound to dextran for the chromatographic purification of most mammalian IgG. Human IgG_3 does not bind to protein A, and DEAE purified IgG was added to the protein A-dextran column and IgG_3 appeared alone in the direct-flowed component