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建立了测定全血中环孢素 A( Cs A) 浓度的受体结合法,并与荧光偏振免疫法( F P I A) 进行比较。用环孢素 A 的重组受体亲环素 A 作为结合蛋白,3 H Cs A 作示踪剂,活性炭吸附分离结合的与游离的两相,测定结合相的放射性。测定了30 例肾移植患者 Cs A 血药浓度并与 F P I A 的结果进行比较。受体结合法的有效测定范围为501600 ngml ,变异系数批内为8 .2 % ( n = 8) ,批间为10 .6 % ( n = 6) ,测定结果与 F P I A 法相关( r= 0 .96 ; P<0 .001) 。测定 Cs A 的受体结合法灵敏、特异、重复性和稳定性好,测定结果与 F P I A 法相关,适用于临床血药浓度的测定。
A receptor binding assay for the determination of CsA concentration in whole blood was established and compared with the fluorescence polarization immunoassay (F P I A). Cyclosporin A recombinant receptor cyclophilin A as a binding protein, 3 H Cs A as a tracer, activated carbon adsorption and separation of bound with free two phases, binding phase radioactivity. The plasma concentration of CsA in 30 renal transplant recipients was measured and compared with that of F P I A. The effective range of the receptor binding assay was 501600 ngml and the variation coefficient was within 8 batches. 2% (n = 8) with 10 between lots. 6% (n = 6). The results were correlated with F P I A (r = 0.96; P <0.001). Sensitivity, specificity, repeatability and stability of CsA receptor binding assay were determined. The results were correlated with F P I A method and could be used to determine the plasma concentration of CsA.