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采用限制性培养技术与温度梯度诱导相结合的方法,从四川成都平原多年还田的土壤中筛选、构建出一组在中温条件下对水稻秸秆具有腐解功能的复合菌系RSS-4。该复合菌系在22℃条件下,稻秆腐解试验表明:pH先升高后降低,最后稳定在7.20;纤维素酶活、半纤维素酶活均经历了先升后降的变化趋势,最高酶活分别为0.91、3.40U;到16d腐解结束时,RSS-4对稻秆、纤维素及半纤维素的降解率分别达到了45.0%、55.5%和44.1%,而木质素在整个腐解过程中未发生明显的变化;说明所筛选构建的这组腐解复合菌系可加速稻秆的腐解。同时发现采用未灭菌的筛选方法筛得的复合菌系RSS-4比灭菌所得的RSS-4′腐解效果要好。
A combination of restriction culture and temperature gradient induction was used to screen a group of composite strains of RSS-4, which had decomposed function to rice stalks at middle temperature, from the soils that had been in the field for many years in the Chengdu Plain, Sichuan Province. Under the conditions of 22 ℃, the composting bacteria decomposing rice straw showed that the pH increased first and then decreased and finally stabilized at 7.20; the activities of cellulase and hemicellulase all went through the first and then decreased trend, The highest enzyme activities were 0.91 and 3.40U, respectively. At the end of 16 d, the degradation rates of rice straw, cellulose and hemicellulose reached 45.0%, 55.5% and 44.1%, respectively, No significant changes were observed in the decomposition process. The results showed that the decomposed strains could accelerate the decomposition of rice straw. At the same time, it was found that the composite strain RSS-4 screened by non-sterilized screening method had a better effect than the sterilized RSS-4 ’.