论文部分内容阅读
对海南霸王岭热带雨林的12科16种常见植物的丛枝菌根状况进行了调查,用碱解离-酸性品红染色法进行了真菌鉴定。结果表明,13种植物形成典型的丛枝菌根,占所调查植物的81%;3种植物没有形成丛枝菌根,占所调查植物的19%。用湿筛沉淀法从这些植物根际土壤中共分离鉴定出了3属11种丛枝菌根真菌(AMF),即无梗囊霉属(Acaulos-pora)3种,球囊霉属(Glomus)7种,巨孢囊霉属(Gigaspora)1种;其中,球囊霉属是样地的优势属。在AMF中,孔窝无梗囊霉(A.foveata)分离频率最高,在14种植物的根际土中都有发现;此外,大果球囊霉(G.macrocarpum)的相对多度最大,为59%,具有最强的产孢能力。同时,在11种植物的根中发现了深色有隔内生真菌(DSE),占调查植物的69%;其中,11种植物同时被DSE和AMF感染。
The arbuscular mycorrhizal status of 12 families of 16 species of common plants in Bawangling Tropical Rainforest of Hainan Island was investigated. The fungi were identified by alkaline dissociation-acid fuchsin staining. The results showed that 13 species of plants formed typical arbuscular mycorrhizae, accounting for 81% of the investigated plants; 3 species did not form arbuscular mycorrhizae, accounting for 19% of the investigated plants. A total of 11 AMF species belonging to 3 genera were identified and isolated from rhizosphere soil of these plants by wet sieving. Three species of Acaulos-pora, three species of Glomus, 7 species and 1 species of Gigaspora. Among them, Glomus was the predominant species in the sample. In AMF, A.foveata was the most frequently isolated in the rhizosphere soils of 14 species. In addition, G.macrocarpum had the highest relative abundance, 59%, with the strongest sporulation capacity. At the same time, dark endophytic fungi (DSE) were found in the roots of 11 plants, accounting for 69% of the investigated plants; 11 of them were infected with both DSE and AMF.