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目的：探讨细胞膜带３蛋白介导的ＨＣＯ－３／Ｃｌ－交换和碳酸酐酶（ＣＡ）催化的ＨＣＯ－３脱水化反应在肺气体交换中的作用。方法：通过对麻醉犬右心房适时地灌注４，４′－二硫氰基－２，２′－二磺基芪（ＤＩＤＳ）抑制带３蛋白、乙酰唑胺（ＡＣＴＺ）抑制红细胞和肺组织ＣＡ活性来完成。结果：①ＤＩＤＳ可导致灌注后１５、３０ｍｉｎ的ＣＯ２产量（Ｖ·ＣＯ２）较灌注前显著减少（Ｐ＜０．０５）、心输出量（Ｑ）显著地增高（Ｐ＜０．０５）。１ｈ后完全恢复正常。②ＡＣＴＺ除出现上述变化且程度较重外，尚出现了灌注后１５、３０ｍｉｎ的耗氧量（Ｖ·Ｏ２）较灌注前显著减少（Ｐ＜０．０５）等改变。其变化在１ｈ后完全恢复正常。③ＤＩＤＳ和ＡＣＴＺ两者之间在Ｖ·ＣＯ２减少、血ＰＣＯ２增高、ｐＨ下降等方面存在协同效应。④正常犬红细胞阴离子交换速率ｋ值在１０℃、ｐＨ７．４１、红细胞数为８．０×１０８个／ｍｌ、介质液为盐酸盐的条件下为（２．２２±０．２）ｈ－１。结论：ＣＡ和／或带３蛋白是影响肺气体交换的重要因素，二者在肺气体交换过程中可能存在某种程度的协同作用。
OBJECTIVE: To investigate the role of membrane protein 3-mediated HCO-3 / Cl-exchange and carbonic anhydrase (CA) -catalyzed dehydration of HCO-3 in lung gas exchange. Methods: Anesthetize the right atrium of anesthetized dog with 4, 4’-dithiocyanato-2,2’-disulfo-stilbene (DIDS) inhibitory zone 3 protein and acetazolamide (ACTZ) to inhibit erythrocyte and lung CA Activity to complete. Results: (1) DIDS induced a significant reduction in CO2 production (V · CO2) at 15 and 30 min after perfusion compared with that before perfusion (P <0.05) and cardiac output (Q) increased significantly (P <0.05). After 1h completely returned to normal. ② In addition to the above changes in ACTZ and the degree of heavier, there is still a 15,30 min after perfusion of oxygen (V · O2) significantly decreased compared with before perfusion (P <0.05) and other changes. The change completely returned to normal after 1h. ③ There is a synergistic effect between DIDS and ACTZ in reducing V · CO2, increasing blood PCO2 and decreasing pH. ④ normal dogs erythrocyte anion exchange rate k value at 10 ℃, pH7.41, the number of red blood cells was 8.0 × 108 / ml, the medium solution of hydrochloric acid was (2.22 ± 0.2) h- 1. CONCLUSION: CA and / or zone 3 proteins are important factors affecting the exchange of lung gas, and there may be some degree of synergy between the two in the process of lung gas exchange.