目的建立畲族的永久性淋巴母细胞株以探讨基因组结构及其健康素质以及遗传结构，同时为畲族纯系基因组的保存提供一种行之有效的手段。方法按照经典的建株方法，以携带ＥＢ病毒的Ｂ９５－８细胞转化畲族青少年的外周血淋巴细胞，其中男性１５例，女性１５例，平均年龄１５．５１±１．０７岁。结果经培养筛选，目前能连续传代的培养物有１２份。染色体观察，未发现核型在数目和结构上有明显变化。结论所建成的这些细胞株可进一步为遗传学及分子生物学的研究提供随时可取的实验材料 Objective To establish a permanent Sheep lymphoblastoid cell line to explore the genomic structure, health quality and genetic structure, and to provide an effective means for the preservation of She s pure line genome. Methods According to the classical method of establishment, peripheral blood lymphocytes were transformed from B95-8 cells carrying Epstein-Barr virus (Epstein-Barr virus), including 15 males and 15 females, with an average age of 15.51 ± 1.07 years. The results of culture selection, the current continuous culture of 12 copies. Chromosome observation, found no significant changes in the number and structure of karyotype. Conclusion The constructed cell lines can further provide ready-to-use experimental materials for genetics and molecular biology research