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目的:建立同时测定胃苏颗粒中柚皮苷、橙皮苷和新橙皮苷含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent Zorbax SB-C18柱,流动相为甲醇-醋酸-水(35∶4∶61,V/V/V),流速为1.0 ml/min,检测波长为283 nm,进样量为5μl,柱温为35℃。结果:柚皮苷、橙皮苷、新橙皮苷检测质量浓度分别在9.413～188.3、4.929～98.59、5.112～102.2μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.999 9);精密度、稳定性、重复性试验的RSD≤1.3%;平均加样回收率分别为101.08%、99.89%、101.46%,RSD分别为0.62%、1.35%、0.45%(n=6)。结论:该方法简便、准确、重复性好,适用于测定胃苏颗粒中3种黄酮苷类成分的含量。
Objective: To establish a method for the simultaneous determination of naringin, hesperidin and neohesperidin in Weisu granules. Methods: Using high performance liquid chromatography. The column was an Agilent Zorbax SB-C18 column with a mobile phase of methanol-acetic acid-water (35:4:61, V / V / V) at a flow rate of 1.0 ml / min with a detection wavelength of 283 nm and an injection volume of 5 μl The column temperature was 35 ° C. Results: The calibration curves of naringin, hesperidin and neohesperidin showed a good linear relationship with the peak areas in the ranges of 9.413 ~ 188.3, 4.929 ~ 98.59 and 5.112 ~ 102.2 μg / ml, respectively (r = 0.999 9 ). The RSD of precision, stability and repeatability test were less than 1.3%. The average recoveries were 101.08%, 99.89% and 101.46%, respectively. The RSDs were 0.62%, 1.35% and 0.45% (n = 6). Conclusion: The method is simple, accurate and reproducible. It is suitable for the determination of three flavonoid glycosides in Wei Su granules.