目的对2012年河南省疟疾流行三类县(以下简称疟疾三类县)专业技术人员的疟疾检测能力进行考核评估,了解其当前的疟原虫检测能力。方法 2012年9~12月,对河南省疟疾三类县(市、区)疾控中心和辖区内医疗机构中相应的专业技术人员进行能力考核评估,考核内容包括疟疾相关基础理论知识(疟疾病原学、临床表现、诊断与治疗和流行病学等基础知识;计满分100分,60分为及格)、血片制作(每位技术人员在1 h内制作4张血片,并吉氏染色;计满分40分,24分为及格)和镜检读片(每位技术人员镜检6张标准血片,每张血片计时8 min,进行定性和虫种鉴别;计满分60分,36分为及格),用SSPS17.0软件分别从技术人员的性别、年龄、职称、单位级别和单位类型等方面对其成绩进行统计学分析。结果共有891名专业技术人员参加考核,成绩合计平均分为162.1分,最高者200分(满分),最低者96分,及格(平均120分以上)人数为871人,占97.8%。不同性别、年龄、职称、单位级别和单位类型人员之间的血片制作成绩差异均无统计学意义(P>0.05)。男性与女性技术人员间的理论成绩和镜检成绩的差异均无统计学意义(P>0.05),女性技术人员的合计成绩(162.97±17.64)高于男性(159.01±20.33)(P<0.05)。>50岁技术人员的理论成绩(84.38±9.41)低于≤30岁(89.91±7.81)和31~40岁(89.96±7.74)(P<0.05);>50岁技术人员的镜检读片成绩(34.62±14.82)和合计成绩(144.62±20.33)均显著低于其他3个年龄组(≤30岁:45.75±13.58和162.50±18.90;31~40岁:46.53±12.72和163.51±17.77;41~50岁:46.22±13.38和159.80±17.32)(P<0.05)。初级、中级和高级职称技术人员的理论成绩(88.33±8.23,90.00±7.76和92.37±7.29)、镜检成绩(44.88±13.62,46.59±12.88和49.57±11.98)和合计成绩(159.61±18.37,163.81±18.03和169.15±16.38)均依次递增,且各组间差异有统计学意义(P<0.05)。乡级、县级和省市级技术人员的理论成绩(88.28±8.30,90.84±7.32,93.54±6.10)、镜检成绩(44.54±13.14,47.69±13.40,52.62±11.04)和合计成绩(159.48±18.33,165.92±17.31,171.97±15.53)均依次递增,且各组间差异有统计学意义(P<0.05)。疾控中心和医疗机构技术人员的各项成绩差异均较小,无统计学意义(P>0.05)。结论河南省疟疾三类县专业技术人员对疟疾诊治的总体水平均衡,需加强对初、中级职称和基层医疗单位技术人员的技能培训。 Objective To assess the malaria detection ability of professional and technical personnel in three types of counties (hereinafter referred to as malaria in three counties) in Henan Province in 2012 to understand the current detection ability of malaria parasites. Methods From September to December 2012, capacity assessment was conducted on the three types of malaria control centers in Henan Province and the corresponding professional and technical personnel in the medical institutions within the jurisdiction. The assessment contents include malaria-related basic theoretical knowledge (malaria pathogen (100 points out of 100, pass of 60 points), blood film production (each technician made 4 blood films in 1 h and stained with Kyrgyzstan; Points out of 40 points, 24 points for the pass) and microscopy readings (each technician microscopic examination of 6 standard blood films, each blood film timing 8 min, qualitative and insects identification; full score 60 points, 36 points As the pass), with SSPS17.0 software, respectively, from the technical staff of gender, age, job title, unit level and unit type and other aspects of their performance statistics. Results A total of 891 professional and technical personnel participated in the examination. The total score was 162.1 points, the highest score was 200 points, the lowest score was 96 points, and the number of eligible students was 871 (97.8%). There were no significant differences in blood film production scores between different genders, ages, job titles, unit level and unit type (P> 0.05). There was no significant difference between the male and female technicians in the theoretical and microscopic examination results (P> 0.05). The total score of female technicians was 162.97 ± 17.64 (159.01 ± 20.33) (P <0.05) . > 50 years old technician’s theoretical score (84.38 ± 9.41) was lower than ≤30 years old (89.91 ± 7.81) and 31 ~ 40 years old (89.96 ± 7.74) (P <0.05); (34.62 ± 14.82) and total score (144.62 ± 20.33) were significantly lower than the other three age groups (≤30 years: 45.75 ± 13.58 and 162.50 ± 18.90; 31-40 years: 46.53 ± 12.72 and 163.51 ± 17.77; 50 years old: 46.22 ± 13.38 and 159.80 ± 17.32) (P <0.05). The theoretical scores (88.33 ± 8.23,90.00 ± 7.76 and 92.37 ± 7.29) for primary, intermediate and senior technicians, the microscopic examination scores (44.88 ± 13.62,46.59 ± 12.88 and 49.57 ± 11.98) and the total score (159.61 ± 18.37,163.81 ± 18.03 and 169.15 ± 16.38), all of which were statistically significant (P <0.05). The theoretical scores (88.28 ± 8.30,90.84 ± 7.32,93.54 ± 6.10), the microscopic examination results (44.54 ± 13.14,47.69 ± 13.40,52.62 ± 11.04) and the total score (159.48 ± 18.33,165.92 ± 17.31,171.97 ± 15.53) all increased in turn, and the differences among the groups were statistically significant (P <0.05). CDC and medical institutions, the technical staff of the differences are small, not statistically significant (P> 0.05). Conclusion The overall level of malaria diagnosis and treatment among professional and technical personnel in three counties of malaria in Henan Province needs to be balanced. Skills training for technicians in primary and secondary professional titles and primary medical units should be strengthened.