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目的:探讨微RNA-98(miRNA-98)和微RNA-143(miRNA-143)在鼻腔鼻窦恶性肿瘤发生、发展及转移中的作用和临床意义。方法:采用实时荧光定量PCR方法检测53例鼻腔鼻窦恶性肿瘤组织、50例鼻息肉组织及20例正常鼻黏膜组织中miRNA-98和miRNA-143的表达量,并分析其在不同临床病理指标下的表达水平。结果:鼻息肉组织及正常鼻黏膜组织中miRNA-98的表达水平显著低于鼻腔鼻窦恶性肿瘤组织(P<0.05)。鼻腔鼻窦恶性肿瘤组织中miRNA-98的表达量和临床分期与淋巴结转移有关,与病理分级无相关。鼻息肉组织及正常鼻黏膜组织中miRNA-143的表达水平显著高于鼻腔鼻窦恶性肿瘤组织(P<0.05)。结论:鼻腔鼻窦恶性肿瘤的发生、发展和转移与miRNA-98的表达呈正相关,与miRNA-143的表达呈负相关。
Objective: To investigate the role and clinical significance of miRNA-98 (miRNA-98) and microRNA-143 (miRNA-143) in the occurrence, development and metastasis of nasal sinus cancer. Methods: The mRNA expression levels of miRNA-98 and miRNA-143 in 53 cases of nasal sinus cancer, 50 cases of nasal polyps and 20 cases of normal nasal mucosa were detected by real-time fluorescence quantitative PCR. The expression of miRNA-98 and miRNA- The level of expression. Results: The expression of miRNA-98 in nasal polyps and normal nasal mucosa was significantly lower than that in nasal sinus malignant tumors (P <0.05). The expression and clinical stage of miRNA-98 in nasal sinus malignant tumors were related to lymph node metastasis, but not to pathological grade. The expression of miRNA-143 in nasal polyps and normal nasal mucosa was significantly higher than that in nasal sinus malignant tumors (P <0.05). Conclusion: The occurrence, development and metastasis of nasal sinus cancer are positively correlated with the expression of miRNA-98 and negatively correlated with the expression of miRNA-143.