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目的探讨喉鳞癌组织中垂体肿瘤转化基因(PTTG)和碱性成纤维细胞生长因子(bFGF)的表达以及在新生血管生成过程中的作用。方法应用免疫组化SP法分别检测58例喉鳞癌组织和30例癌旁组织中PTTG和bFGF两种蛋白的表达情况,并用CD34标记并计数微血管密度(MVD)。结果 PTTG和bFGF在喉鳞癌组织中的表达分别为72.41%和84.48%,均高于癌旁组织的33.33%和36.67%(P<0.05);bFGF和PTTG的表达与喉鳞癌淋巴结转移、分化程度及临床分期均密切相关(P<0.05),且两者在喉鳞癌组织中表达呈显著正相关(P<0.05)。MVD值在喉鳞癌组织中表达明显大于喉鳞癌旁黏膜(P<0.05),并与喉鳞癌淋巴结转移呈显著相关(P<0.05)。MVD分别与喉鳞癌组织中PTTG和bFGF表达呈正相关(P<0.05)。结论 PTTG和bFGF在喉鳞癌组织中的高表达与MVD关系密切,在喉鳞癌发展中可能起重要作用。两者联合检测有助于喉鳞癌的诊断和恶性程度评估。
Objective To investigate the expression of pituitary tumor transforming gene (PTTG) and basic fibroblast growth factor (bFGF) in laryngeal squamous cell carcinoma and its role in neovascularization. Methods The expressions of PTTG and bFGF in 58 cases of laryngeal squamous cell carcinoma and 30 cases of adjacent normal tissues were detected by immunohistochemical SP method, and the microvessel density (MVD) was marked by CD34. Results The expressions of PTTG and bFGF in laryngeal squamous cell carcinoma were 72.41% and 84.48%, respectively, which were significantly higher than those in para-cancerous tissues (33.33% vs 36.67%, P <0.05). The expressions of bFGF and PTTG were correlated with lymph node metastasis, (P <0.05). There was a significant positive correlation between the differentiation and clinical stage (P <0.05). MVD was significantly higher in laryngeal squamous cell carcinoma than in laryngeal squamous cell carcinoma (P <0.05), and significantly correlated with lymph node metastasis (P <0.05). MVD was positively correlated with the expression of PTTG and bFGF in laryngeal squamous cell carcinoma (P <0.05). Conclusion The high expression of PTTG and bFGF in laryngeal squamous cell carcinoma is closely related to MVD, which may play an important role in the development of laryngeal squamous cell carcinoma. Joint detection of the two can help the diagnosis of laryngeal squamous cell carcinoma and the evaluation of the degree of malignancy.