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目的:建立高效液相色谱法分析甜叶菊中3个甜菊醇糖苷含量。方法:采用Poroshell 120 EC-C18色谱柱(4.6 mm×150mm,2.7μm),以乙腈-磷酸水溶液(30∶70)为流动相进行分离,在紫外检测波长210 nm下进行检测,外标法定量。结果:3个甜菊醇糖苷的检出限为1.0~1.2 mg·kg-1,定量限为2.5~3.0 mg·kg-1;在50~1000 mg·L-1的浓度范围内线性良好,相关系数为0.9999~1.000;瑞鲍迪苷A、瑞鲍迪苷C和甜菊苷的加标回收率分别为92.8%、87.2%、90.3%,RSD分别为3.9%、2.7%、4.8%;精密度的RSD分别为1.2%、2.1%、1.3%;重复性的RSD分别为3.3%、6.4%和4.7%。结论:本方法操作简便,快速,分离度和准确度高,可用于甜叶菊原料的质量控制。
Objective: To establish a HPLC method for the determination of three steviol glycosides in Stevia rebaudiana. METHODS: Poroshell 120 EC-C18 column (4.6 mm × 150 mm, 2.7 μm) was used to separate the sample with acetonitrile-phosphoric acid solution (30:70) as mobile phase and UV detection wavelength was set at 210 nm. . Results: The detection limits of three steviol glycosides were 1.0-1.2 mg · kg-1 with the limits of quantitation of 2.5-3.0 mg · kg-1. The linearity was good in the range of 50-1000 mg · L-1 The coefficients of calibration curve were 0.9999-1.000. The recoveries of rebaudioside A, rebaudioside C and stevioside were 92.8%, 87.2% and 90.3%, respectively. The RSDs were 3.9%, 2.7% and 4.8%, respectively. The precision The RSDs were 1.2%, 2.1% and 1.3% respectively. The repeatability RSDs were 3.3%, 6.4% and 4.7% respectively. Conclusion: The method is simple, rapid, high resolution and accuracy and can be used for the quality control of stevia.