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目的:探讨甘附冲剂抗大鼠心动过缓的可能作用机制。方法:用普萘洛尔(Pro)制备大鼠心率减慢模型;测定大鼠心肌组织中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量。放射免疫法测定心肌组织中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)及血浆中降钙素基因相关肽(CGRP)、内皮素(ET)的含量。结果:30,90,270mg.kg-1甘附冲剂可显著抑制Pro引起的大鼠心率减慢(P<0.05或P<0.01),和心肌组织中MDA含量的升高,提高大鼠血浆CGRP的含量,并可显著降低心肌组织中cGMP的含量,提高cAMP/cGMP比值(P<0.05或P<0.01);90,270 mg.kg-1甘附冲剂可增强大鼠心肌组织中SOD和GSH-PX的活性(P<0.05);甘附冲剂各剂量对大鼠心肌组织中cAMP含量及大鼠血浆ET含量并没有明显影响。结论:甘附冲剂抗大鼠心动过缓的作用可能与抗自由基、升高CGRP及调节cAMP和cGMP的平衡有关。
Objective: To investigate the possible mechanism of Ganfu granule in preventing bradycardia in rats. Methods: Rat model of heart rate reduction was established by propranolol (Pro). The activity of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) Aldehyde (MDA) content. The contents of cAMP, cGMP, CGRP and ET in myocardium were measured by radioimmunoassay. Results: 30,90,270 mg.kg-1 Ganfu granule could significantly inhibit the slowing down of Pro-induced rat heart rate (P <0.05 or P <0.01), increase the content of MDA in myocardial tissue and increase the level of CGRP (P <0.05 or P <0.01). The concentration of cGMP in myocardial tissue and the ratio of cAMP / cGMP in myocardium were significantly increased by 90,270 mg.kg-1 Ganfu granules Activity (P <0.05). The dose of Ganfu granules had no significant effect on cAMP content in rat myocardium and ET content in rat plasma. Conclusion: The effect of Ganfu granules on bradycardia in rats may be related to anti-free radical, increasing CGRP and regulating the balance of cAMP and cGMP.