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目的 探索ＣＤ５７＋ 自然杀伤细胞（ＮＫ细胞，ｎａｔｕｒａｌｋｉｌｌｅｒｃｅｌｌｓ）在小鼠妊娠早期子宫壁内的意义。方法 用小鼠抗人ＮＫ细胞ＣＤ５７单克隆抗体，对未经产小鼠妊娠早期子宫壁内ＮＫ细胞进行免疫组织化学标记。结果 孕０ｄ，小鼠子宫壁内见少量ＣＤ５７＋ 细胞，孕２ｄ 时ＮＫ细胞数量剧增，孕４ｄ 时稍下降。孕６ｄ、孕８ｄ 时数量更少（低于０ｄ 值）。孕６ｄ 和８ｄ 均出现妊娠失败个体，其子宫壁内ＮＫ细胞很多，显著高于正常妊娠６ｄ 和８ｄ 小鼠的数量（Ｐ＜ ０．０１）。从分布区域看，胚胎着床（４．５ｄ）前，ＮＫ细胞多位于子宫肌层，胚胎着床后仅存在于着床点以外的子宫内膜和肌层。在妊娠失败个体，ＮＫ细胞广泛存在于子宫内膜和肌层中。 结论 小鼠交配后子宫ＮＫ细胞增多与子宫炎性反应有关。着床期以后子宫ＮＫ 细胞增多与妊娠失败有一定的相关性。
Objective To explore the significance of CD57 + natural killer cells (NK cells) in early stage of uterine wall in mice. Methods The mouse anti-human NK cell CD57 monoclonal antibody was used to immunohistochemically label the NK cells in uterine wall of unproductive mice. Results Pregnant 0d, a small number of CD57 + cells in the mouse uterine wall, the number of NK cells dramatically increased on the 2nd day of pregnancy, slightly decreased on the 4th day of pregnancy. 6d pregnant, 8d pregnant when the number is less (less than 0d value). Pregnant 6d and 8d pregnant individuals have failed to pregnancy, the wall of the uterus in a lot of NK cells, significantly higher than the number of normal pregnancy 6d and 8d mice (P <0.01). From the distribution area, before embryo implantation (4.5d), NK cells mostly located in the myometrium, and only existed in the endometrium and muscularis outside the implantation site after implantation. In pregnancy failure individuals, NK cells are widely found in the endometrium and muscle. Conclusion Mice uterine NK cells increased after mating and uterine inflammatory response. Implantation of uterine NK cells increased after pregnancy and pregnancy have a certain correlation.