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本文报道,通过联合应用放射性碘标记,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),蛋白免疫印染和细胞表面单克隆抗体(McAb)等方法,进一步鉴定百日咳杆菌毒株UT25、无毒株UT25D和UT25NA的加热改性的外膜蛋白(OMP)的均一性及其分布情况。结果表明,UT25的OMP粗制品、经35℃以上处理,SDS-PAGE显示OMP分子量为91000(91K)和32K,经60℃以上溶解作OMP分子量为38K和30K。某些OMP的蛋白带经较低温度处理后清晰可见,但经较高温度处
This paper reports the combination of radioactive iodine labeling, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and cell surface monoclonal antibody (McAb) and other methods to further identify the Bordetella pertussis strain UT25, non-toxic UT25D and UT25NA heat-modified outer membrane protein (OMP) uniformity and distribution. The results showed that the crude OMP of UT25 was treated at above 35 ℃. The molecular weight of OMP was 91000 (91K) and 32K by SDS-PAGE, and the molecular weights of OMP were 38K and 30K after being dissolved at 60 ℃. Some OMP protein bands are clearly visible at lower temperatures, but at higher temperatures