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目的:研究特异性环氧化酶-2(COX-2)抑制剂NS-398和生长抑素类似物奥曲肽联合使用对人肝癌细胞系SMMC-7721增殖、凋亡的影响。方法:采用四氮唑盐比色法(MTT法)观察细胞增殖活力改变,流式细胞仪检测细胞凋亡百分率。结果:(1)MTT法显示NS-398和奥曲肽均在一定范围内呈浓度和时间依赖性地抑制SMMC-7721细胞的增殖;联合用药组抑制率显著高于单用NS-398或奥曲肽组(P<0.01),金正均方法显示q>1.15,提示两药联合应用有协同抑制肝癌细胞增殖效应,并随着作用时间延长而增强。(2)流式细胞仪测定显示,NS-398、奥曲肽和两药联合作用于SMMC-7721细胞24 h后,联合用药组细胞凋亡率显著高于单一用药组(P<0.01)。结论:NS-398和奥曲肽呈剂量、时间依赖性地抑制SMMC-7721细胞增殖,促进SMMC-7721细胞凋亡,两者联合应用具有协同作用。
OBJECTIVE: To study the effects of combined cyclooxygenase-2 (COX-2) inhibitor NS-398 and somatostatin analogue octreotide on the proliferation and apoptosis of human hepatoma cell line SMMC-7721. Methods: The cell viability was observed by MTT method. The percentage of apoptosis was detected by flow cytometry. Results: (1) MTT assay showed that NS-398 and octreotide both inhibited the proliferation of SMMC-7721 cells in a concentration-and time-dependent manner in a certain range. The inhibition rate of combination group was significantly higher than that of NS-398 or octreotide alone group P <0.01), Kim Jung-kun method showed that q> 1.15, suggesting that the combination of the two drugs synergistically inhibited the proliferation of hepatocellular carcinoma cells and increased with time. (2) The results of flow cytometry showed that the apoptotic rates of NS-398, Octreotide and the combination of two drugs in SMMC-7721 cells for 24 h were significantly higher than that of single drug group (P <0.01). Conclusion: NS-398 and octreotide can inhibit the proliferation of SMMC-7721 cells in a time-and dose-dependent manner and promote the apoptosis of SMMC-7721 cells in a dose-and time-dependent manner. The combination of NS-398 and octreotide has a synergistic effect.