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取 Wistar 大鼠生后1～3天乳鼠的心肌组织,用0.06%的胰蛋白酶制备细胞悬液,在含15%小牛血清的培养液中培养30小时后用于实验。在倒置显微镜的保温罩内观察心肌细胞团的搏动。用一个自制装置,将因细胞搏动产生的光强度变化转换成电讯号,经放大后记录于多导生理仪上。实验按药物浓度分组,每组细胞样品均在10个以上。对照组经连续观察15～30分,未见搏动频率和振幅有明显变化。当去甲乌药碱的最终浓度为10~(-6)～10~(-3)M 时,能明显增快细胞的搏动频率,以10~(-4)～10~(-5) M的作用最明显,分别从129±5.5及122±6.6次/分增
Myocardial tissue of the suckling rat was harvested 1 to 3 days after birth in Wistar rats. The cell suspension was prepared with 0.06% trypsin and cultured for 30 hours in a medium containing 15% fetal bovine serum for experiment. Inspect the pulsation of the cardiomyocyte mass in a thermostated cover of an inverted microscope. Using a self-made device, the change in light intensity due to the pulsation of the cells is converted into an electrical signal which is amplified and recorded on a multi-guide physiology instrument. The experiment grouping by drug concentration, each group of cell samples are more than 10. The control group after continuous observation of 15 to 30 minutes, no significant fluctuations in pulsatile frequency and amplitude. When the final concentration of naringin was 10 ~ (-6) ~ 10 ~ (-3) M, the pulsatile frequency of cells could be obviously increased and the rate of 10 ~ (-4) ~ 10 ~ (-5) M The most obvious effect, respectively, from 129 ± 5.5 and 122 ± 6.6 times / min increase