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采用实验室模拟的方法研究了氟啶胺对土壤过氧化氢酶活性、动力学和热力学特征参数的影响。结果表明:氟啶胺对土壤过氧化氢酶表现出不同程度的抑制作用,氟啶胺浓度越大,对土壤过氧化氢酶活性的抑制作用越显著。不同浓度氟啶胺胁迫下土壤过氧化氢酶酶促反应的Km值和Vmax值发生改变。在100 mg/kg氟啶胺胁迫下,土壤过氧化氢酶对底物的亲和力最小,而CK中过氧化氢酶对底物的亲和力最大。温度对过氧化氢酶活性影响较复杂,高浓度(100、1 000 mg/kg)氟啶胺胁迫下过氧化氢酶活性变化幅度较小,低浓度(1、5、10、50 mg/kg)氟啶胺胁迫下过氧化氢酶活性变化幅度相对较小。其中50 mg/kg氟啶胺胁迫下过氧化氢酶活性变化最大,307 K下酶活性最大,为1.277 3 mL/kg,277 K下酶活性最小,为1.085 6 mg/kg。
The effects of fluazinam on soil catalase activity, kinetic and thermodynamic parameters were studied by laboratory simulation. The results showed that: fluazinam showed different degrees of inhibition of soil catalase, the greater the concentration of fluazinam, the more significant inhibition of soil catalase activity. The Km value and Vmax value of soil catalase under different concentrations of fludiazine stress changed. Under 100 mg / kg of fluazinam, soil catalase had the lowest affinity to substrate, while catalase in CK had the highest affinity to substrate. The effects of temperature on the activities of catalase were complicated. The changes of catalase activity under the concentration of 100 and 1 000 mg / ) The change of catalase activity under fluaziamid stress was relatively small. Among them, the activity of catalase changed most under the stress of 50 mg / kg fluazinam and the activity was the highest at 307 K, which was 1.277 3 mL / kg. The enzyme activity was the lowest at 277 K, 1.085 6 mg / kg.