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研究了谷子(Setaria italica(L.)Beauv.)在从拔节期开始进行不同氮供应水平处理(1.88、15mmol·L-1)和从抽穗期到灌浆期进行增强UV-B辐射处理(24.7μW·cm-2)条件下叶片主要氮代谢指标的变化。结果表明:1)在抽穗初期,氮供应水平对游离氨基酸含量和谷氨酰胺合成酶(GS)活性无显著影响,高氮供应水平下谷子叶硝态氮含量、谷氨酸脱氢酶(GDH)和硝酸还原酶(NR)活性均显著上升(p<0.05);2)在灌浆末期,氮供应水平和增强UV-B辐射对硝态氮含量和NR活性均无显著影响,高氮供应水平显著提高了增强UV-B辐射处理条件下GS和GDH活性(p<0.05)。研究认为,较高氮供应水平更有利于提高增强UV-B辐射处理下谷子叶片在主要繁殖阶段的氮代谢水平。
The effects of different nitrogen supply levels (1.88,15mmol·L-1) and enhanced UV-B radiation treatment (24.7μW) from heading stage to filling stage were studied in the field of Setaria italica (L.) Beauv. · Cm-2). The results showed that: 1) At the early heading stage, the nitrogen supply had no significant effect on free amino acid content and glutamine synthetase (GS) activity. Under high nitrogen supply, nitrate nitrogen content, glutamate dehydrogenase ) And nitrate reductase (NR) activity increased significantly (p <0.05); 2) At the end of filling stage, nitrogen supply and enhanced UV-B radiation had no significant effect on nitrate nitrogen content and NR activity, Significantly increased GS and GDH activities under enhanced UV-B radiation treatment (p <0.05). The study suggests that the higher nitrogen supply level is more conducive to enhancing the level of nitrogen metabolism of millet leaves during the main breeding stage under enhanced UV-B radiation treatment.