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目的研究胃癌组织中VEGF-C和VEGFR-3表达与淋巴结转移及患者预后关系。方法1994年4月至2003年12月手术切除胃癌组织石蜡标本,按是否有淋巴结转移分为2组。用VEGF-C、VEGFR-3单克隆抗体DAB法免疫组化染色,用Kaplan-Meier分析生存率,二分变量回归和Cox回归分析其对患者预后的影响。结果188例胃癌中,无淋巴结转移者[N(-)组]91例,有淋巴结转移者[N(+)组]97例,N(-)组VEGF-C、VEGFR-3阳性率低于N(+)组。在N(-)组与N (+)组、VEGF-C表达阴性组与阳性组之间平均淋巴管密度差异有统计学意义。VEGF-C和VEGFR- 3表达阳性和阴性组的5年累积生存率差异有统计学意义。结论结果表明VEGF-C、VEGFR-3表达与淋巴结转移有关,影响胃癌患者预后。胃癌组织中VEGF-C、VEGFR-3可预测淋巴结转移,是评估胃癌患者预后的重要指标之一。
Objective To study the relationship between the expression of VEGF-C and VEGFR-3 in gastric carcinoma and lymph node metastasis and prognosis. Methods From April 1994 to December 2003, paraffin specimens of gastric cancer tissues were divided into two groups according to whether there was lymph node metastasis. The VEGF-C and VEGFR-3 monoclonal antibodies were immunohistochemically stained with DAB. Kaplan-Meier analysis of survival rate, dichotomous variable regression and Cox regression analysis of the prognosis of patients. Results The positive rate of VEGF-C and VEGFR-3 in 97 gastric cancer patients without lymph node metastasis [n (-) group], 97 with lymph node metastasis [N (+) group] N (+) group. There was significant difference in average lymphatic vessel density between N (-) group and N (+) group, VEGF-C negative group and positive group. The 5-year cumulative survival rates of VEGF-C and VEGFR-3 positive and negative groups were statistically different. Conclusion The results show that the expression of VEGF-C and VEGFR-3 is related to lymph node metastasis, which affects the prognosis of patients with gastric cancer. VEGF-C and VEGFR-3 in gastric cancer can predict lymph node metastasis, which is one of the important indexes to evaluate the prognosis of gastric cancer.