论文部分内容阅读
目的:探讨人子宫内膜腺上皮细胞和基质细胞中雌、孕激素对Pbx2蛋白表达的影响。方法:采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)连接法检测人增生期、分泌中期和蜕膜期子宫内膜中Pbx2的表达和分布;体外培养人子宫内膜基质细胞和高分化子宫内膜癌细胞Ishikawa,分别加入雌激素、孕激素、雌、孕激素联合刺激48 h,并以不加雌、孕激素的基质细胞核和Ishikawa细胞为对照组,采用免疫组织化学、免疫印迹法测定各种条件下Ishikawa细胞中Pbx2蛋白的表达。结果:①在人各期子宫内膜组织中,Pbx2表达于腺上皮细胞和基质细胞的细胞核中;Pbx2在分泌中期和蜕膜期基质细胞中的表达显著高于增生期,但在腺上皮细胞中增生期组织的表达高于分泌中期和蜕膜期,差异均具有统计学意义(P<0.05)。②Western blotting结果显示,在孕激素和雌、孕激素联合处理Ishikawa细胞组中,Pbx2的表达显著低于对照组(P<0.05),雌激素处理后Pbx2的表达与其他各组间的表达无显著性差异(P>0.05),在人子宫内膜基质细胞(ESC)中,Pbx2的表达在雌、孕激素处理各组间比较均无统计学差异(P>0.05)。结论:在人子宫内膜腺上皮Ishikawa细胞中孕激素对Pbx2的表达具有下调作用,在人子宫内膜基质细胞中,Pbx2的调控可能是非雌、孕激素依赖性的。
Objective: To investigate the effects of estrogen and progesterone on the expression of Pbx2 in human endometrial glandular epithelial cells and stromal cells. Methods: The expression and distribution of Pbx2 in the endometrium of human during proliferative phase, secretory phase and decidual phase were detected by immunohistochemical streptavidin-peroxidase (SP) ligation. Human endometrial stromal Cells and well-differentiated endometrial carcinoma cells Ishikawa, respectively, were added estrogen, progesterone, estrogen and progesterone combined stimulation 48 h, and without estrogen and progesterone stromal nucleus and Ishikawa cells as control group, immunohistochemistry The expression of Pbx2 protein in Ishikawa cells was determined by immunoblotting. Results: (1) Pbx2 was expressed in the nucleus of glandular epithelial cells and stromal cells in all stages of human endometrium. The expression of Pbx2 in stromal cells at mid-secretory phase and decidual phase was significantly higher than that in proliferative phase, but in glandular epithelial cells The expression of mesangial cells in the proliferative phase was higher than that in the metaphase and decidua, both of which were statistically significant (P <0.05). Western blotting showed that the expression of Pbx2 in Ishikawa cells treated with progesterone and estrogen and progesterone was significantly lower than that in control group (P <0.05). The expression of Pbx2 in estrogen-treated Ishikawa cells was not significantly different from other groups (P> 0.05). The expression of Pbx2 in human endometrial stromal cells (ESC) was not significantly different between estrogen and progesterone treatment groups (P> 0.05). CONCLUSION: Progesterone can down-regulate the expression of Pbx2 in human endometrial glandular epithelial Ishikawa cells. In human endometrial stromal cells, the regulation of Pbx2 may be non-estrogen and progestin-dependent.