论文部分内容阅读
用灵敏的夹心酶联免疫法（ＥＬＩＳＡ）建立了检测重组人ｈＧＭ－ＣＳＦ药代动力学的方法。此方法ｈＧＭ～ＣＳＦ在１２．５～０．３９ｎｇ·ｍｌ￣（－１）范围内，检测呈线性相关。最低检测灵敏度为０．４ｎｇ·ｍｌ￣（－１）。本方法不受血清中潜在干扰因素的影响，检测具高度特异性。大鼠ｓｃｈＧＭ－ＣＳＦ５０，１００，２００μｇ·ｋｇ￣（－１）后１５ｍｉｎ，血中即有较高浓度的ｈＧＭ－ＣＳＦ，１ｈ左右达峰浓度，以后快速下降。皮下给药，尿中可检测到原型ｈＧＭ－ＣＳＦ，但累积排泄率较低。本研究为临床合理用药提供依据。
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect the pharmacokinetics of recombinant human hGM-CSF. This method hGM ~ CSF in the range of 12.5 ~ 0.39ng · ml ~ (-1), the test was linear correlation. The lowest detection sensitivity of 0.4ng · ml ~ (-1). The method is not affected by the potential interference in serum, the detection of a high degree of specificity. Rats with schGM-CSF50,100,200μg · kg ~ (-1) after 15min, the blood that have a higher concentration of hGM-CSF, peaked at about 1h concentration, then decreased rapidly. Subcutaneously, the prototype hGM-CSF was detectable in urine but with a low cumulative excretion rate. This study provides the basis for clinical rational drug use.