作者用非水反相高压液相层析法成功地分离亲脂性维生素D_2和D_3。使用Zorbax-ODS柱,1%甲醇-乙腈流动相。本法将预定量的维生素D_3内标加到部分片粉样品中,然后用二甲亚砜、水、甲醇和己烷的混合液提取。浓缩部分的已烷提取液通过二氧化硅柱分路纯化,除去干扰物质。以维生素D_2作为内标时,本法 The authors successfully isolated lipophilic vitamin D 2 and D 3 using non-aqueous RP-HPLC. A Zorbax-ODS column, 1% methanol-acetonitrile mobile phase was used. This method will be a predetermined amount of vitamin D_3 internal standard added to part of the sample powder, and then with dimethyl sulfoxide, water, methanol and hexane mixture of extraction. The concentrated hexane extract was purified by silica column to remove interfering substances. With vitamin D_2 as an internal standard, this law