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本文应用维甲酸(RA)对肺巨细胞癌高转移株(PLA-801-D)进行了诱导分化研究,结果显示:诱导后PLA-801-D细胞出现成熟分化的特征。细胞生长曲线下降,分裂指数减少,倍增时间延长;细胞体积增大,呈铺砖样排列。电镜下,细胞器及中丝增多,找到张力原纤维,细胞连接增多等成熟分化特征。免疫组化染色,细胞角蛋白与波形蛋白均为阳性,说明肿瘤细胞可能是较幼稚的上皮细胞。癌基因DNA-mRNA斑点杂交显示:诱导后的细胞v-src墓因表达增加,N-ras基因表达下降。证实这两种基因在肿瘤的恶性转化中可能起一定作用。
In this paper, we used retinoic acid (RA) to study the differentiation of pulmonary giant cell carcinoma high metastasis strain (PLA-801-D). The results showed that the induction of differentiation of PLA-801-D cells after induction. The cell growth curve decreased, the mitotic index decreased, and the doubling time prolonged; the cell volume increased and paved like a brick. Under the electron microscope, the organelles and the silk increased, and the tensile fibrils were found and the cell connection increased. Immunohistochemical staining showed that both cytokeratin and vimentin were positive, suggesting that the tumor cells may be more naive epithelial cells. Oncogene DNA-mRNA dot blot hybridization showed that after the induction of v-src cells, the expression of toxin increased and the expression of N-ras gene decreased. It was confirmed that these two genes may play a role in the malignant transformation of tumors.