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独活(Angelicae Pubescentis Radix)作为一种被广泛应用的中药,常用于治疗风湿性疾病.本研究通过运用UPLC-Q-TOF-MS液质联用技术定性分析独活提取物中的化学成分.独活提取物的分离通过Agilent SB-C18色谱柱(1.8μm,2.1 mm×50 mm)经梯度洗脱完成,流动相由0.1％甲酸-水和乙腈组成.通过配有ESI电离源的质谱仪在m/z 100-800全扫描正离子模式下进行数据采集.通过比较一级和二级质谱数据,共鉴定出49个化合物,包括46个香豆素类和3个非香豆素类化合物.其中2个化合物被鉴定为新化合物,isoangenomalin,scoparone,4-methyl-umbelliferyl acetate,suberenol,trans-dehydroosthol和oroselone为首次在独活中发现的化合物.“,”Angelicae Pubescentis Radix (APR,Duhuo) is a commonly used traditional Chinese medicine and usually used for the treatments of rheumatic diseases.To assess the chemical composition of APR extract,a sensitive and reliable UPLC-Q-TOF-MS method was used for qualitative analysis.The separation was achieved on an Agilent SB-C18 column (1.8 μm,2.1 mm×50 mm)with a gradient elution system consisting of acetonitrile and water containing 0.1％ formic acid.An electrospray ionization (ESI) was used for mass spectrometer,and the data were collected in the positive ion mode,which was operated in a full-scan mode at m/z 100-800.A total of 49 compounds including 46 coumarins were identified according to the MS and MS/MS data.Among them,two were new compounds,and isoangenomalin,scoparone,4-methyl-umbelliferyl acetate,suberenol,trans-dehydroosthol,and oroselone were first reported in APR.