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利用陆地棉遗传背景的海岛棉染色体16置换系材料Sub16和陆地棉多基因标记系T586创建了含1259个单株的F2作图群体,结合本实验室最新的栽培四倍体棉种种间分子遗传图谱上染色体16的标记信息,利用分子标记技术,对红株基因R1进行精细定位.在F2分离群体中,红株性状分离比符合孟德尔1:2:1的分离,进一步证明该性状是由一不完全显性单基因控制.利用JoinMap3.0连锁分析软件,使用含237个单株的F2小群体完成红株基因R1的初级定位,进一步利用含1259个单株的F2大群体将该基因精细定位在NAU4956和NAU6752之间,与最近标记的遗传距离为0.49cM.研究结果为进一步克隆该基因及培育红色彩棉转基因品种提供了研究基础.
In this study, F2 population of 1259 individuals was established by using IS16 cotton chromosome 16 substitution line material (Sub16) and Gossypium hirsutum (Gossypium hirsutum L.) multi-gene marker line T586. Based on the latest genetic mapping of tetraploid cotton The marker information of chromosome 16 was mapped on chromosome 16 and the red gene R1 was finely mapped by molecular marker technique.In the F2 segregation population, the segregation ratio of red traits was 1: 2: 1 with Mendelian segregation, which further proved that the trait was An incomplete dominant single-gene control.Using JoinMap3.0 linkage analysis software, the F2 locus containing 237 individuals were used to complete the primary locus of red gene R1 and the F2 population of 1259 individuals was further used to map the gene The fine mapping was between NAU4956 and NAU6752 and the genetic distance to the nearest marker was 0.49cM.The research provided the basis for further cloning this gene and cultivating red colored cotton transgenic lines.