论文部分内容阅读
目的:研究大蒜素是否具有药物性预处理的作用,并探讨蛋白激酶C在大蒜素预处理中的重要作用。方法:34只离体家兔心脏挂上Langendorff装置,以95％O_2/5％CO_2混合的Krebs-Henseleit液灌流,均接受30min局部缺血和2h复灌。结果:在30min局部缺血前10min予5min药物性预处理的大蒜素组较之对照组明显缩小心肌梗塞范围(7％±6％ vs25％±7％,P<0.05),大蒜素+多粘菌素B组的心肌梗塞范围与对照组相比无显著性差异(23％±5％ vs 25％±7％,P>0.05)。结论:大蒜素具有模拟缺血预处理样保护作用,其作用可被抑制剂多粘菌素B所阻断,表明大蒜素通过激活蛋白激酶C发挥药物性预处理作用。
Objective: To study whether allicin has the effect of drug pretreatment and to explore the important role of protein kinase C in allicin pretreatment. METHODS: Thirty-four isolated rabbit hearts were hung on a Langendorff device and perfused with Krebs-Henseleit solution mixed with 95% O 2 /5% CO 2 , and both received ischemia for 30 min and 2 h for irrigation. RESULTS: The allicin preconditioning group was significantly reduced in the myocardial infarction range (7% ± 6% vs25% ± 7%, P<0.05), and the allicin + polyviscosity was pretreated 5 min before the 30 min ischemia. There was no significant difference in myocardial infarction range between Bactin group B and control group (23%±5% vs 25%±7%, P>0.05). Conclusion: Allicin has the protective effect of simulated ischemic preconditioning. The effect of allicin can be blocked by inhibitor polymyxin B, indicating that allicin exerts drug pretreatment by activating protein kinase C.