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本试验通过黑麦草和苇状羊茅的杂交、杂种和黑麦草的回交以及杂种细胞遗传学的研究表明:四倍体多花黑麦草和苇状羊茅杂交所获种子发芽率极低,只有通过幼胚培养才能获得较多的杂种苗。苇状羊茅和黑麦草间种质的转移是有细胞学基础的;[L.multiflorum(4x)×F.arundinacea(6x)]F_1杂种植株与四倍体多花黑麦草回交以及与二倍体多年生黑麦草杂交时,可育雌配子染色体数分别约是n=28-35和n=13-24;从黑麦草育种的角度出发,四倍体多花黑麦草和苇状羊茅F_1杂种用四倍体多花黑麦草回交的效果比F_1杂种自交、F-1杂种和二倍体多年生黑麦草杂交的效果好;二倍体黑麦草和苇状羊茅F_1杂种回交结实率与回交后代的自交结实率极低不可能获得较大的群体,就这点而论,通过这种F_1(2n=28)和黑麦草回交进行黑麦草育种的途径效率较低。二倍体多花黑麦草×苇状羊茅和二倍体多年生黑麦草×苇状羊茅F_1杂种经秋水仙素处理后均获得了双二倍体(2n=56)。多花黑麦草(2n=28,14)和苇状羊茅F_1杂种植株田间越夏情况介于双亲之间。
In this study, the cross between the ryegrass and the tall fescue, the backcrosses of the hybrids and the ryegrass, and the hybrid cytogenetic studies showed that the germination rate of the seeds obtained from the cross of the tetraploid ryegrass and the tall fescue was extremely low, Only through immature embryo culture to get more hybrid seedlings. The transfer of germplasm between L. fescue and ryegrass was cytologically based [L.multiflorum (4x) × F.arundinacea (6x)] F_1 hybrid plants were backcrossed with tetraploid ryegrass and with two The chromosome numbers of fertile female gametes were about n = 28-35 and n = 13-24, respectively, when they were crossed with perennial ryegrass. From the perspective of breeding of ryegrass, tetraploid ryegrass and fescue F_1 Hybrids with tetraploid ryegrass backcrossing effect than F_1 hybrids self-cross, F-1 hybrids and diploid perennial ryegrass cross hybrid effect is good; diploid ryegrass and fescue F_1 hybrid backcross In this regard, the low efficiency of ryegrass breeding through this F_1 (2n = 28) backcrossing with ryegrass is low. Diploid diploid (2n = 56) were obtained after treatment with colchicine, diploid ryegrass × tall fescue and diploid perennial ryegrass × tall fescue F_1 hybrids, respectively. The more summer ryegrass (2n = 28,14) and the F 1 hybrid fescue were between the parents.