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目的:探讨不同盐浓度对盐敏感高血压肾脏纤维化及及巨噬细胞浸润的影响。方法:将6周龄Dahl盐敏感大鼠随机分为正盐组(0.3%氯化钠)、4%氯化钠组、8%氯化钠组三组,每组15只,持续喂养8周。在8周时间点应用tail-cuff method方法监测鼠尾血压,Masson三原色法检测三组大鼠肾脏组织纤维化情况,免疫组化及Western blotting方法检测各组大鼠肾脏巨噬细胞浸润情况。结果:1)4%高盐组及8%高盐组大鼠8周时间点血压均较正盐组现在升高,8%高盐组大鼠血压较4%高盐组进一步升高。2)4%高盐组及8%高盐组大鼠8周时间点肾脏相对重量及肾脏纤维化程度较正盐组增加,8%高盐组较4%高盐组进一步增加。3)4%高盐组及8%高盐组大鼠8周时间点肾脏组织巨噬细胞浸润增加,8%高盐组较4%高盐组进一步增加。结论:盐浓度不同对盐敏感性肾脏纤维化及巨噬细胞浸润程度影响不同,盐浓度升高可加重盐敏感高血压时肾脏纤维化及及巨噬细胞浸润。
Objective: To investigate the effects of different salt concentrations on renal fibrosis and macrophage infiltration in salt-sensitive hypertensive rats. Methods: Six-week-old Dahl salt-sensitive rats were randomly divided into three groups: normal saline group (0.3% sodium chloride), 4% sodium chloride group and 8% sodium chloride group, . Tail blood pressure was measured by tail-cuff method at 8 weeks. Masson’s three primary colorimetric method was used to detect renal tissue fibrosis in rats. Immunohistochemistry and Western blotting were used to detect the renal macrophage infiltration. Results: 1) The blood pressure of rats in 4% high salt group and 8% high salt group was higher than that of normal saline group at 8 weeks, and that of 8% high salt group was higher than that of 4% high salt group. 2) The relative weight of kidney and the degree of renal fibrosis in 4% high-salt group and 8% high-salt group rats at 8 weeks were higher than those in normal saline group and those in 8% high-salt group and 4% high-salt group. (3) The macrophage infiltration in renal tissue increased in rats in 4% high salt group and 8% high salt group at 8 weeks, and further increased in 8% high salt group compared with 4% high salt group. Conclusion: Different salt concentrations have different effects on salt-sensitive renal fibrosis and macrophage infiltration. Increased salt concentration may aggravate renal fibrosis and macrophage infiltration in salt-sensitive hypertension.