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应用细胞培养和流式细胞仪分析技术分析全蝎水煎液对HL60细胞增殖、细胞形态及细胞凋亡的影响。结果全蝎水提物对HL60细胞增殖有一定的抑制作用,细胞凋亡比例由对照组的736%增加至947%(4mg/ml)、1256%(8mg/ml)和2144%(12mg/ml)。细胞形态表现为细胞增大,核膜皱缩,核固缩或核碎裂,但细胞膜完整。表明全蝎可诱导HL60细胞凋亡,并对细胞增殖有一定的抑制作用
Cell culture and flow cytometry analysis techniques were used to analyze the effects of decoction of whole peony on the proliferation, cell morphology and apoptosis of HL-60 cells. RESULTS: The total water extract had a certain inhibitory effect on the proliferation of HL-60 cells, and the proportion of apoptosis increased from 7.36% in the control group to 9.47% (4 mg/ml) and 12.56% (8 mg/ml). ) and 21 44% (12 mg/ml). The cell morphology showed an increase of cells, nuclear membrane shrinkage, nuclear condensation or nuclear fragmentation, but the cell membrane was intact. Shows that whole quail can induce apoptosis of HL 60 cells, and has a certain inhibitory effect on cell proliferation