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对不同来源的１０个品种的黄瓜种子低温下萌发研究结果表明，种子萌发最敏感的低温是１３～１５℃。在此温度范围内，不同品种黄瓜种子发芽率有显著差异。低温萌发过程中，ＰＯＤ活性均呈曲线变化，有一高峰出现，并且按高峰出现时间的早晚划分成３种类型。高峰出现早的品种，其种子发芽率高，萌发速度快；反之，发芽率低，萌发速度慢。低温抑制了ＰＯＤ活性，减少了ＰＯＤ同功酶条带和强度。
The results of germination studies on cucumber seeds from 10 different cucumber sources at different temperatures showed that the most sensitive low temperature for seed germination was 13-15 ℃. In this temperature range, different varieties of cucumber seed germination rates were significantly different. During the process of low temperature germination, POD activity showed a curve change, with a peak appeared, and divided into three types according to the peak appearance time. Early peak appeared varieties, the seed germination rate is high, germination rate; the other hand, low germination rate, germination rate is slow. Low temperature inhibits POD activity and reduces POD isoenzyme bands and intensities.