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目的探讨一组正反互补单克隆抗体对胃癌患者腹腔冲洗液中脱落癌细胞检测的应用价值。方法应用低分子量细胞角蛋白(CKLMW)、癌胚抗原(CEA)、间皮细胞(MC)及波形蛋白(Vim)4种标志物,对67例胃癌患者腹腔冲洗液中的癌细胞成分进行免疫细胞化学(ICC)检测,并与常规细胞病理学(CP)检测结果比较。结果ICC阳性检出率(86.2%)明显优于CP阳性诊断率(58.6%)(P<0.05);ICC阳性检出率随侵袭深度及病期进展而增加;CKLMW、CEA、MC及Vim4种抗体的可用度分别为64.6%、71.6%、77.9%、41.8%。结论联合应用CKLMW、CEA及MC对于检测胃癌腹腔冲洗液中脱落癌细胞具有重要的临床价值,而Vim缺乏诊断意义。
Objective To investigate the value of a set of positive and negative complementary monoclonal antibodies in the detection of exfoliated cancer cells in peritoneal washings of patients with gastric cancer. Methods Four kinds of markers, CKLMW, CEA, MC and Vim, were used to immunize the cancer cells in the peritoneal washings of 67 patients with gastric cancer Cytochemistry (ICC) test and compared with routine cytopathology (CP) test results. Results The ICC positive rate (86.2%) was significantly higher than that of CP (58.6%) (P <0.05). The positive rate of ICC increased with the invasion depth and disease progression. CKLMW, CEA, MC and Vim The availability of antibodies were 64.6%, 71.6%, 77.9% and 41.8%, respectively. Conclusions The combined application of CKLMW, CEA and MC has important clinical value for the detection of shedding cancer cells in peritoneal washings of gastric cancer, while Vim lacks diagnostic significance.