论文部分内容阅读
用体内和体外试验的方法观察ＮａＦ对大鼠脑突触体膜Ｃａ￣（２＋）Ｍｇ￣（２＋）－ＡＴＰ酶活性的影响。ＮａＦ（０．３、１．６、８．０、２０．０、４０．０ｍｍｏｌ／Ｌ）明显抑制该酶活性，抑制串分别为６．６％、１８．０％、４１．０％、５５．５％和６３．１％，半数抑制浓度为１４．８ｍｍｏｌ／Ｌ，存在明确的剂量效应与时间效应关系。酶底物动力学分析表明，ＮａＦ的这种效应属非况争性抑制。在妊娠期和哺乳期饮用５、１５和５０ｍｇ／Ｌ加氟水５０天后，母鼠脑突触体膜Ｃａ￣（２＋）Ｍｇ￣（２＋）－ＡＴＰ酶活性有降低趋势，其子鼠在１５和５０ｍｇ／Ｌ组该酶活性抑制达１１．３％和３２．１％。
The effects of NaF on Ca ~ (2+) Mg ~ (2 +) - ATPase activity in rat brain synaptosomes were observed in vitro and in vivo. NaF (0.3, 1.6, 8.0, 20.0, 40.0 mmol / L) significantly inhibited the activity of the enzyme and the inhibitory effect was 6.6%, 18.0%, 41.0%, 55 .5% and 63.1%, respectively. The median inhibitory concentration was 14.8mmol / L, and there was a clear dose-effect relationship with time effect. Kinetic analysis of the enzyme substrate showed that this effect of NaF is an uncompromised inhibition. The activity of Ca ~ (2+) Mg ~ (2 +) - ATPase in the synaptosomal membrane of female rats decreased after drinking 5, 15 and 50 mg / L fluoride water for gestational and lactation. The enzyme activity was inhibited by 11.3% and 32.1% in the 50 mg / L group.