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目的:观察不同剂量BCG-LPS在不同干预时间对哮喘小鼠气道高反应性及气道炎症的干预作用。方法:将Balb/c雌鼠随机分为哮喘模型组、NS对照组和BCG-LPS干预组,根据干预的时间和干预制剂剂量的不同将干预组分为:-7LPS1μg、-7LPS10μg、-7LPS100μg、10LPS1μg、10LPS10μg、10LPS100μg、17LPS1μg、17LPS10μg、17LPS100μg组;在末次激发48 h后,测定各浓度级乙酰甲胆碱激发下的增强的呼气间歇(EnhancedPause,Penh)值,将其与小鼠激发前吸入NS后的Penh的百分比(Penh%NS)作为其气道反应性评价指标,并对肺泡灌洗液进行细胞学分析。结果:(1)气道反应性:-7LPS1μg组在Mch为25-50 mg/ml之间的Penh%NS显著低于哮喘组(P<0.05);-7LPS10μg、17LPS10μg和NS组在Mch为3.12-50 mg/ml之间的Penh%NS,显著低于哮喘组(P<0.05);-7LPS100μg和17LPS100μg组在Mch为12.5-50 mg/ml之间的Penh%NS显著低于哮喘组(P<0.05)。(2)气道炎症:-7LPS10μg组的BALF Eos%显著低于哮喘组;-7LPS10μg组的BALF Eos%显著低于-7LPS1μg、-7LPS100μg、10LPS10μg和17LPS10μg组(P<0.05)。结论:BCG-LPS能降低哮喘小鼠的气道高反应性,减轻哮喘小鼠的气道炎症,早期(-7天)中小剂量的干预效果较佳。
OBJECTIVE: To observe the intervention effects of different dosages of BCG-LPS on airway hyperresponsiveness and airway inflammation in asthmatic mice at different times of intervention. Methods: The Balb / c female mice were randomly divided into asthma model group, NS control group and BCG-LPS intervention group. According to the time of intervention and the dosage of intervention preparation, the intervention groups were divided into 7LPS1μg, -7LPS10μg, -7LPS100μg, 10LPS1μg, 10LPS10μg, 10LPS100μg, 17LPS1μg, 17LPS10μg, 17LPS100μg group; After the last 48 hours, the enhanced concentration of methacholine-induced enhancedPhoshopause (EnhancedPause, Penh) value, and the mouse before the challenge The percentage of Penh after inhalation of NS (Penh% NS) was used as an index of airway responsiveness, and the cytology of the alveolar lavage fluid was analyzed. Results: (1) Airway reactivity: The Penh% NS of -7 LPS1 μg group was significantly lower than that of asthma group (P <0.05) at Mch of 25-50 mg / ml; in MLC of 3.12 Penh% NS between -50 mg / ml was significantly lower than that of the asthma group (P <0.05); Penh% NS in the LPS100μg and 17LPS100μg groups was significantly lower than that of the asthma group (P <0.05). (2) Airway inflammation: BALF Eos% of -7LPS10μg group was significantly lower than asthma group; BALF Eos% of -7LPS10μg group was significantly lower than -7LPS1μg, -7LPS100μg, 10LPS10μg and17LPS10μg group (P <0.05). CONCLUSION: BCG-LPS can reduce the airway hyperresponsiveness in asthmatic mice and alleviate the airway inflammation in asthmatic mice. The effect of early and middle-dose (-7 days) intervention is better.